Microbiotests-Algaltoxkit-F-freshwater-algae-toxicity-test

Algaltoxkit F

Order code TK41

The freshwater algae toxicity test is intended for toxicity screening of chemicals, effluents, surface waters, wastewaters, groundwaters, sediment pore waters and elutriates.

Algaltoxkit F contains all the materials you need to perform two 72h growth inhibition tests with the freshwater microalgae Pseudokirchneriella subcapitata (former names: Selenastrum capricornutum and Raphidocelis subcapitata). This cost-effective and culture-independent Algal toxicity test strictly adheres to ISO Standard 8692 and OECD Guideline 201.

Download the Algaltoxkit F product list.

ORDER Algaltoxkit F

Order now

  • This field is for validation purposes and should be left unchanged.
Link to PDF

FRESHWATER ALGAE TOXICITY TEST

Test criterion

The freshwater algae toxicity test is a 72h bioassay based on growth inhibition of the freshwater microalgae Pseudokirchneriella subcapitata, with calculation of the 72h EC50.

Learn more about the freshwater algae toxicity test procedure

Contents of the Algaltoxkit F

  • Tubes with algal beads which you can de-immobilise on demand in less than 30 min to supply the live biota for the freshwater algae toxicity test.
  • Concentrated algal growth and toxicant dilution medium, long cell cuvettes and holding trays.
  • Tubes with algal beads which you can de-immobilise on demand in less than 30 min to supply the live biota for the freshwater algae toxicity test.
  • Concentrated algal growth and toxicant dilution medium, long cell cuvettes and holding trays.
  • Short Bench Protocol and standard operating procedure brochure with easy-to-follow instructions and detailed illustrations.
  • OD/N regression sheet, data scoring sheets and a Quality Control specification sheet with batch number of the algal beads and the media.

All test materials and equipment included in the Algaltoxkit F are available separately.

Benefits of freshwater algae toxicity test

  • We produce high-quality algal beads in strictly controlled conditions. This way, we preclude variability associated with recruitment/maintenance of live stocks of microalgae in exponential growth phase in conventional bioassays.
  • The quality of the test medium is highly uniform, thanks to simple dilution of concentrated solutions of selected chemicals with deionised water.
  • A Quality Control test with a reference chemical is described in detail, so you can easily check accuracy and reproducibility.
  • The Algaltoxkit F uses unique disposable “long cell” exposure chambers (10 cm path length), which serve as measuring cuvettes for direct determination of algal growth by optical density measurements.
  • If you store the ephippia properly, you can prolong their shelf life for several months and reduce test scheduling constraints.
  • The freshwater algae toxicity test requires minimal space and equipment:
  • tabletop centrifuge
  • spectrophotometer
  • incubator with lateral lights
  • conventional laboratory glassware
  • If you store the algal beads properly, you can prolong their shelf life for several months and reduce test scheduling constraints.
  • Simple handlings and scorings.
  • You can start the freshwater algae toxicity test within 30 min after you’ve de-immobilised the microalgae from the algal beads.
  • Daily scoring of the optical density of the microalgae in the long cells takes less than 15 min.
  • We can provide you with a computer program for easy Toxkit data treatment.

The sensitivity of the Algaltoxkit F toxicity test is comparable to the sensitivity of the conventional algal growth inhibition test with microalgae from laboratory cultures.

  • The freshwater Algaltoxkit F is used in many environmental laboratories and research institutes worldwide and is validated by extensive international interlaboratory comparisons.
  • You can find a substantial number of publications, posters, reports and reviews for various uses.
  • Our freshwater algae toxicity test complies with the protocols ISO Standard 8692 and OECD Guideline 201, for regulatory testing with microalgae.

Interested in buying Algaltoxkit F?

It contains all necessary materials to perform two 72h growth inhibition tests with the freshwater microalgae Pseudokirchneriella subcapitata.