BIOLIGHT Aliivibrio fischeri
Acute toxicity reagents and luminometer
BioLight reagents are applicable for the luminescent bacteria toxicity test using the freeze dried bacteria Aliivibrio fischeri (formerly known as Vibrio fischeri ) which has bioluminescent properties.
The inhibition of light emitted by the luminescent bacteria is the test criterion for measuring toxicity.
The BioLight Toxy all-in-one luminometer measures the light levels of Aliivibrio fischeri to determine the toxicity of a sample.
The BioLight Acute Toxicity Reagents are standardized for use with bioassay methods described in ISO 11348-3 and ISO 21338.
Luminescent bacteria test
with aliivibrio fischeri
Aliivibrio fischeri (formerly Vibrio fischeri ) is a marine, Gram negative and non-pathogen bacterium that luminesces as a natural part of its metabolism under optimal environmental conditions.
When exposed to a toxic substance, the metabolic respiratory process of this bioluminescent bacterium is disrupted, reducing light output. This inhibition of luminescence by Aliivibrio fischeri can be measured after 5-30 minutes with a photometer (luminometer).
The reduction of light intensity measured, directly correlates with the degree of toxicity of the sample relative to the control sample.
The application of freeze-dried luminescent bacteria proves to be a useful tool for the assessment and monitoring of toxicity.
The testing combination of the BioLight Reagents and consumables with the BioLight Toxy allows for screening of 3600 chemical compounds simultaneously.
Bioassays using microbial biosensors are rapid, sensitive, reproducible and user-friendly..
Luminescent bacteria test method is applicable for the screening of:
chemicals
industrial effluent and process water
aqueous extracts and leachates from hazardous waste
eluates of sediment and contaminated soil
monitoring of fresh, marine and brackish water
drinking water monitoring
drilling mud or fluids
…
The bioluminescence inhibition bioassay with Aliivibrio fischeri ( syn. Vibrio fischeri ) has been standardized (luminescence bacteria test ISO 11348-3 and ISO 21338) and is widely used for a variety of ecotoxicological applications and research for more than 30 years.
ORDER INFORMATION
- order code BIO2019L BioLight Toxy – Bench Top
Includes : BioLight Instrument with on board PLC and software for both field and lab protocols.
Cooling block to hold 12 mm cuvettes with USB connection to the unit for power
Battery for up to 8 hours use in the field. Wall plug for inside usage. Rugged carrying case. - order code BIO2018P BioLight Toxy – Portable
Includes : BioLight Instrument with on board PLC and software for field protocols.
Battery for up to 8 hours use in the field. Wall plug for inside usage. Rugged carrying case. - order code BIO2018A Upgrade from BioLight Portable to Bench Top
Includes : Cooling block to hold 12 mm cuvettes with USB connection to the unit for power.
USB for adding lab protocols to the software - order code BIO2020 Cooling Block for 12 mm Tubes
Includes : Cooling block to hold 12 mm cuvettes with USB connection to the unit for power.
This item can only be acquired when the user has purhased BIO2019L or BIO2018A. - order code BIO2021 Cooling Block for 17 mm Tubes (Solids Testing)
Includes : Cooling block to hold 17 mm cuvettes with USB connection to the unit for power.
This item can only be acquired when the user has purhased BIO2019L or BIO2018A.
- order code BIO2006 BioLight Multi Test, Freeze Dried Bacteria (10 vials/box)
- order code BIO2007 BioLight Single Test, Freeze Dried Bacteria (50 vials/box)
- order code BIO2001 BioLight Recon (50 mL)
- order code BIO2002 BioLight Diluent (1 L)
- order code BIO2003 BioLight Solid Phase Diluent (1 L)
- order code BIO2004 BioLight Salinity Adjusting Solution (50 mL)
- order code BIO2005 BioLight Recon (1 L)
- order code BIO2017 BioLight Tri-Salt Diluent (1 L)
- item code BIO2015A Disposable Glass Cuvettes (200)
- order code BIO2016A Disposable Glass Cuvettes (1000)
- order code RAW1035 10-100 µl Pipet Tips
- order code RAW1036 100-1000 µl Pipet Tips
- order code BIO2010 Extended Length Tips for Solid Phase
- order code BIO2011 Solid Phase Filter Column and Tubes (1000)
BioLight bioluminescent bacteria (reagent) is freeze-dried for extended life then stored frozen at -18°C to -20°C and is reconstituted before use. A single vial of reagent contains approximately one hundred million cells. The sample salinity is adjusted to ensure a saltwater atmosphere due to Aliivibrio fischeri being a marine bacterium. Diluent is used for dilutions as required for the test protocol.
BioLight reagents and diluents can be used with commercially available luminometer systems (Microtox®, TOXmini, LumiStox, TOXAlert, …).
Luminescent bacteria tests can be performed with field portable, bench-top and on-line analyzers.
BioLight Acute Toxicity Reagents and Biolight Toxy
Microbiotests distributes the BioLight Toxy and BioLight Reagents from the company Aqua Science , USA.
The Aqua Science team has 40+ years combined experience in the manufacturing of acute toxicity and other custom reagents used for water quality testing. Their highly trained employees include preeminent experts in Aliivibrio fischeri production, so they can offer the highest quality option for toxicity reagent and consumables to this specific market.
The BioLight Toxy luminometer along with the BioLight Reagents and Consumables brings rapid toxicity testing to 21st century.
Their experience includes overseeing the transfer of production from the original method developer (Azur Environmental / Microbics) in 2000, production and product improvement for 11 years at Strategic Diagnostics (SDIX) and at Modern Water for 8 years.
In 2020, this entire team brought their specialized skills and comprehensive knowledge of Aliivibrio fischeri to Aqua Science where they developed BioLight, an improved formula that performs better than any competition in the market.