Test Biota

Test Biota

Aquatic and terrestrial test biota


Aquatic and terrestrial test biota are used worldwide in acute and chronic toxicity tests.  These ecotoxicological bioassays provide qualitative and quantitative data on adverse effects from chemicals or environmental pollutants on living organisms.

Microbiotests provides a wide range of aquatic and terrestrial test biota that can be used for standardised and culture-independent toxicity testing , bioassay services, or for other research applications.
The biota can also be used as starter culture to maintain live stocks or as back-up in the culturing facility of environmental laboratories.

Various freshwater and marine organisms are available: daphnia, algae, rotifers, crustaceans, protozoans, macrophytes (duckweed) and higher plants.

The test biota are not provided as living cultures as such, but as dormant or immobilised test species that can be stored for months and activated (= hatched, de-immobilised or germinated) at the time of the performance of the ecotoxicological bioassays.
Depending on the test species we guarantee a shelf life of the biological material up to 6 months

Many of these test species are identical to the test organisms used in standardised toxicity tests.
As an example, the test biota are used to study whole effluent toxicity tests under national permit and regulatory frameworks.

Test Biota

Microbiotests test species


Microbiotests provides following aquatic and terrestrial test biota as described in International Standards and Guidelines for environmental toxicity testing.


CladoceranDaphnia magnaephippiaISO 6341

OECD 202

OECD 211

US EPA 2021.0

Ceriodaphnia dubiaephippiaUS EPA 2002.0TB35
CrustaceanArtemia franciscanacystsTB32
Thamnocephalus platyuruscystsISO 14380TB31
Heterocypris incongruenscystsISO 14371TB36
RotiferBrachionus calycifloruscystsISO 19827

ISO 20666

Brachionus plicatiliscystsISO 19820TB22
ProzoaTetrahymena thermophilainoculumTB11
AlgaePseudokirchneriella subcapitata

syn. Selenastrum capricornutum

syn. Raphidocelis subcapitata

alginate beadsISO 8692

OECD 201

US EPA 1003.0

Phaeodactylum tricornutuminoculumISO 10253TB42
DuckweedSpirodela polyrhizaturionISO 20227TB73
Higher plantsLepidium sativumseedISO 18763TB61
Sinapis albaseedISO 18763TB62
Sorghum saccharatumseedISO 18763TB63

The intensive culturing of aquatic test organisms and the production of dormant eggs or immobilised test species is one of the key activities and expertise of Microbiotests.

Microbiotests certifies the highest quality of the biological material produced.  All test organisms are cultured in-house under strictly controlled conditions resulting in standardised organisms for reliable toxicity testing.  Every single batch of test biota is subject to thorough internal quality control screenings and reference tests according to international standards and guidelines to guarantee the biological viability and quality of the test species.

Protozoan stocks

TB11   5 vials with Tetrahymena thermophila (freshwater tests)

Rotifer cysts

TB21   10 tubes with Brachionus calyciflorus cysts (freshwater tests)

TB22   10 tubes with Brachionus plicatilis cysts (estuarine / marine tests)

Crustacean cysts

TB31   10 tubes with Thamnocephalus platyurus cysts (freshwater tests)

TB32   10 tubes with Artemia franciscana cysts (estuarine / marine tests)

Daphnia ephippia

TB33   10 tubes with Daphnia magna ephippia (freshwater tests)

TB35   10 tubes with Ceriodaphnia dubia ephippia (freshwater tests)

Ostracoda cysts

TB36   10 tubes with Heterocypris incongruens cysts

Algae beads / inoculum

TB41   5 tubes with Selenastrum capricornutum beads (freshwater tests) + de-immobilisation medium

TB42   5 tubes with Phaeodactylum tricornutum inoculum (estuarine / marine tests)

Higher plants seeds

TB61   10 tubes with sorgho seed (Sorgum saccharatum)

TB62   10 tubes with garden cress seed (Lepidium sativum)

TB63   10 tubes with mustard seed (Sinapis alba)

Duckweed turions

TB73   5 tubes with Spirodela polyrhiza turions