Test Biota

Aquatic and terrestrial test biota


Aquatic and terrestrial test biota are used worldwide in ecotoxicological studies.  These bioassays provide qualitative and quantitative data on adverse effects from chemicals or environmental pollutants on living organisms in acute and chronic toxicity tests.

Microbiotests provides a wide range of aquatic and terrestrial test biota that can be used for standardised and culture-independent toxicity testing , bioassay services, or for other research applications.
These freshwater, marine and estuarine biota can also be used as starter culture to maintain live stocks or as back-up in the culturing facility of environmental laboratories.

Many of these test species are identical to the test organisms used in standardised toxicity tests.
As an example, the test biota are used to study whole effluent toxicity tests under national permit and regulatory frameworks.

The intensive culturing of aquatic test organisms and the production of dormant eggs or immobilised test species is one of the key activities and expertise of Microbiotests.

Microbiotests certifies the highest quality of the biological material produced.
All test organisms are cultured in-house under strictly controlled conditions resulting in standardised organisms
for reliable toxicity testing and research.
Every single batch of test biota is subject to thorough internal quality control screenings and reference tests according to international standards and guidelines to guarantee the biological quality and viability of the test species.

Microbiotests test species


Microbiotests provides following aquatic and terrestrial test biota as described in International Standards and Guidelines for environmental toxicity testing and chemical risk assessment.


Bacteria Aliivibrio fischeri

syn.Vibrio fischeri

freeze dried ISO 11348-3



Cladoceran Daphnia magna ephippia ISO 6341

OECD 202

OECD 211

US EPA 2021.0

Ceriodaphnia dubia ephippia US EPA 2002.0 TB35
Crustacean Artemia franciscana cysts TB32
Thamnocephalus platyurus cysts ISO 14380 TB31
Heterocypris incongruens cysts ISO 14371 TB36
Rotifer Brachionus calyciflorus cysts ISO 19827

ISO 20666

Brachionus plicatilis cysts ISO 19820 TB22
Prozoa Tetrahymena thermophila inoculum TB11
Algae Pseudokirchneriella subcapitata

syn. Selenastrum capricornutum

syn. Raphidocelis subcapitata

alginate beads ISO 8692

OECD 201

US EPA 1003.0

Phaeodactylum tricornutum inoculum ISO 10253 TB42
Duckweed Spirodela polyrhiza turion ISO 20227 TB73
Higher plants Lepidium sativum seed ISO 18763 TB61
Sinapis alba seed ISO 18763 TB62
Sorghum saccharatum seed ISO 18763 TB63
African Turquoise Killifish Nothobranchius furzeri embryonic

diapause eggs


order information

Freeze dried bacteria Aliivibrio fischeri (formerly known as Vibrio fischeri)

BIO2006   BioLigth Multi Test, Freeze Dried Bacteria (10 vials/box)
BIO2007   BioLigth Single Test, Freeze Dried Bacteria (50 vials/box)

Protozoan stocks

TB11   5 vials with Tetrahymena thermophila (freshwater tests)

Rotifer cysts

TB21   10 tubes with Brachionus calyciflorus cysts (freshwater tests)

TB22   10 tubes with Brachionus plicatilis cysts (estuarine / marine tests)

Crustacean cysts

TB31   10 tubes with Thamnocephalus platyurus cysts (freshwater tests)

TB32   10 tubes with Artemia franciscana cysts (estuarine / marine tests)

Daphnia ephippia

TB33   10 tubes with Daphnia magna ephippia (freshwater tests)

TB35   10 tubes with Ceriodaphnia dubia ephippia (freshwater tests)

Ostracoda cysts

TB36   10 tubes with Heterocypris incongruens cysts

Algae beads / inoculum

TB41   5 tubes with Selenastrum capricornutum beads (freshwater tests) + de-immobilisation medium

TB42   5 tubes with Phaeodactylum tricornutum inoculum (estuarine / marine tests)

Higher plants seeds

TB61   10 tubes with sorgho seed (Sorgum saccharatum)

TB62   10 tubes with garden cress seed (Lepidium sativum)

TB63   10 tubes with mustard seed (Sinapis alba)

Duckweed turions

TB73   5 tubes with Spirodela polyrhiza turions

Nothobranchius furzeri embryonic diapause eggs

TB81   100 eggs of Nothobranchius furzeri strain ‘GRZ’