Rotoxkit F chronic

Rotoxkit F chronic

Order code TK23

The chronic, cyst-based rotifer toxicity test is intended for toxicity screening of chemicals, effluents and wastewaters, sediment pore waters and elutriates, surface and groundwaters.

In Rotoxkit F chronic, you find all the materials you need to perform three chronic 48h reproduction tests with the freshwater rotifer Brachionus calyciflorus. This small-scale, cost-effective, culture-independent, user-friendly and highly standardised bioassay complies to ISO Standard 20666.

Download the Rotoxkit F Chronic product list.

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FRESHWATER CHRONIC ROTIFER TOXICITY TEST

Test criterion

The chronic freshwater rotifer toxicity test is a 48h bioassay based on the reproduction of the test organisms, with calculation of the 48h EC50.

Learn more about the rotifer toxicity test procedure

Contents of the Rotoxkit F chronic

  • Tubes with cysts (dormant eggs), which you can hatch on demand within 20h to supply the live biota for the Brachionus calyciflorus toxicity test.
  • Concentrated hatching and toxicant dilution medium, live microalgae immobilised in algal beads, dry inert food, hatching and test containers and micropipettes for the transfer of the organisms.
  • Tubes with cysts (dormant eggs), which you can hatch on demand within 20h to supply the live biota for the Brachionus calyciflorus toxicity test.
  • Concentrated hatching and toxicant dilution medium, live microalgae immobilised in algal beads, dry inert food, hatching and test containers and micropipettes for the transfer of the organisms.
  • Short Bench Protocol and standard operating procedure brochure with easy-to-follow instructions and detailed illustrations.
  • Data scoring sheets and a Quality Control specification sheet with batch number of the cysts and the media.

All test materials and equipment included in the Rotoxkit F chronic are available separately.

Benefits of the rotifer toxicity test

  • We produce high-quality cysts in strictly controlled conditions. This way, we preclude variability associated with recruitment/maintenance of live stocks in conventional bioassays.
  • The quality of the standardised test medium is highly uniform, thanks to simple dilution of concentrated solutions of selected salts with deionised water.
  • A Quality Control test with a reference chemical is described in detail, so you can easily check accuracy and reproducibility.
  • You can hatch the cysts on demand, which eliminates the need and the costs of continuous culturing and maintenance of your test organisms.
  • The freshwater rotifer toxicity test requires minimal space and equipment:
  • dissection microscope
  • incubator with lateral lights
  • conventional laboratory glassware
  • If you store the cysts properly, you can prolong their shelf life for several months and reduce test scheduling constraints.
  • Live algal food for the Brachionus calyciflorus is easily obtained from algal beads included in the kit.
  • Simple handlings and scorings.
  • Specially designed test wells for easy and rapid transfer of the test organisms under a dissecting microscope.
  • Total performance time of the Brachionus calyciflorus toxicity assay is approximately 1 hour.
  • We can provide you with a computer program for easy Toxkit data treatment.
  • Brachionus calyciflorus is quite sensitive to particular chemicals and mixtures.
  • The freshwater Rotoxkit F chronic is used in many environmental laboratories and research institutes worldwide
  • You can find a substantial number of publications, posters, reports and reviews for various uses.
  • Our freshwater chronic rotifer toxicity test strictly adheres to the principles of ISO Standard 20666 for regulatory testing with rotifers.

Interested in buying Rotoxkit F chronic?

It contains all materials necessary to perform three chronic 48h reproduction tests with the freshwater rotifer Brachionus calyciflorus.