Rotoxkit F chronic
The chronic, cyst-based rotifer toxicity test is intended for toxicity screening of chemicals, effluents and wastewaters, sediment pore waters and elutriates, surface and groundwaters.
In Rotoxkit F chronic, you find all the materials you need to perform three chronic 48h reproduction tests with the freshwater rotifer Brachionus calyciflorus. This small-scale, cost-effective, culture-independent, user-friendly and highly standardised bioassay complies to ISO Standard 20666.
How to order?
FRESHWATER CHRONIC ROTIFER TOXICITY TEST
Test criterion
The chronic freshwater rotifer toxicity test is a 48h bioassay based on the reproduction of the test organisms, with calculation of the 48h EC50.
Contents of the Rotoxkit F chronic
- Tubes with cysts (dormant eggs), which you can hatch on demand within 20h to supply the live biota for the Brachionus calyciflorus toxicity test.
- Concentrated hatching and toxicant dilution medium, live microalgae immobilised in algal beads, dry inert food, hatching and test containers and micropipettes for the transfer of the organisms.
- Short Bench Protocol and standard operating procedure brochure with easy-to-follow instructions and detailed illustrations.
- Data scoring sheets and a Quality Control specification sheet with batch number of the cysts and the media.
- Tubes with cysts (dormant eggs), which you can hatch on demand within 20h to supply the live biota for the Brachionus calyciflorus toxicity test.
- Concentrated hatching and toxicant dilution medium, live microalgae immobilised in algal beads, dry inert food, hatching and test containers and micropipettes for the transfer of the organisms.
- Short Bench Protocol and standard operating procedure brochure with easy-to-follow instructions and detailed illustrations.
- Data scoring sheets and a Quality Control specification sheet with batch number of the cysts and the media.
All test materials and equipment included in the Rotoxkit F chronic are available separately.
Benefits of the rotifer toxicity test
- We produce high-quality cysts in strictly controlled conditions. This way, we preclude variability associated with recruitment/maintenance of live stocks in conventional bioassays.
- The quality of the standardised test medium is highly uniform, thanks to simple dilution of concentrated solutions of selected salts with deionised water.
- A Quality Control test with a reference chemical is described in detail, so you can easily check accuracy and reproducibility.
- You can hatch the cysts on demand, which eliminates the need and the costs of continuous culturing and maintenance of your test organisms.
- The freshwater rotifer toxicity test requires minimal space and equipment:
- dissection microscope
- incubator with lateral lights
- conventional laboratory glassware
- If you store the cysts properly, you can prolong their shelf life for several months and reduce test scheduling constraints.
- Live algal food for the Brachionus calyciflorus is easily obtained from algal beads included in the kit.
- Simple handlings and scorings.
- Specially designed test wells for easy and rapid transfer of the test organisms under a dissecting microscope.
- Total performance time of the Brachionus calyciflorus toxicity assay is approximately 1 hour.
- We can provide you with a computer program for easy Toxkit data treatment.
- Brachionus calyciflorus is quite sensitive to particular chemicals and mixtures.
- The freshwater Rotoxkit F chronic is used in many environmental laboratories and research institutes worldwide
- You can find a substantial number of publications, posters, reports and reviews for various uses.
- Our freshwater chronic rotifer toxicity test strictly adheres to the principles of ISO Standard 20666 for regulatory testing with rotifers.
Interested in buying Rotoxkit F chronic?
It contains all materials necessary to perform three chronic 48h reproduction tests with the freshwater rotifer Brachionus calyciflorus.
TOXKIT EQUIPMENT
Dissection microscope
All-round Toxkit incubator
