Acute toxicity reagents
BioLight reagents are applicable for the luminescent bacteria toxicity test using Aliivibrio fischeri (formerly known as Vibrio fischeri ) which has bioluminescent properties.
The inhibition of light emitted by the luminescent bacteria is the test criterion for measuring toxicity.
The BioLight Acute Toxicity Reagents are standardized for use with bioassay methods described in ISO 11348-3 and ISO 21338.
Luminescent bacteria test
with aliivibrio fischeri
Aliivibrio fischeri (formerly Vibrio fischeri ) is a marine, Gram negative and non-pathogen bacterium that luminesces as a natural part of its metabolism under optimal environmental conditions.
When exposed to a toxic substance, the metabolic respiratory process of this bioluminescent bacterium is disrupted, reducing light output. This inhibition of luminescence by Aliivibrio fischeri can be measured after 5-30 minutes with a photometer (luminometer). The reduction of light intensity measured, directly correlates with the degree of toxicity of the sample relative to the control sample.
The application of freeze-dried luminescent bacteria proves to be a useful tool for the assessment and monitoring of toxicity.
Bioassays using microbial biosensors are rapid, sensitive, reproducible and user-friendly..
Luminescent bacteria test method is applicable for the screening of:
industrial effluent and process water
aqueous extracts and leachates from hazardous waste
eluates of sediment and contaminated soil
monitoring of fresh, marine and brackish water
drinking water monitoring
drilling mud or fluids
The bioluminescence inhibition bioassay with Aliivibrio fischeri ( syn. Vibrio fischeri ) has been standardized (luminescence bacteria test ISO 11348-3 and ISO 21338) and widely used for a variety of ecotoxicological applications and research for more than 30 years.
BioLight Acute Toxicity Reagent and Consumables
Microbiotests distributes the BioLight Reagents from the company Aqua Science , USA.
The Aqua Science team has 40+ years combined experience in the manufacturing of acute toxicity and other custom reagents used for water quality testing. Their highly trained employees include preeminent experts in Aliivibrio fischeri production, so they can offer the highest quality option for toxicity reagent and consumables to this specific market.
Their experience includes overseeing the transfer of production from the original method developer (Azur Environmental / Microbics) in 2000, production and product improvement for 11 years at Strategic Diagnostics (SDIX) and at Modern Water for 8 years.
In 2020, this enitre team brought their specialized skills and comprehensive knowledge of Aliivibrio fischeri to Aqua Science.
Where they developed BioLight, an improved formula that performs better than any competition in the market.
- order code BIO2006 BioLight Multi Test, Freeze Dried Bacteria (10 vials/box)
- order code BIO2007 BioLight Single Test, Freeze Dried Bacteria (50 vials/box)
- order code BIO 2001 BioLight Recon (50 mL)
- order code BIO 2002 BioLight Diluent (1 L)
- order code BIO 2003 BioLight Solid Phase Diluent (1 L)
- order code BIO 2004 BioLight Salinity Adjustment (50 mL)
- order code BIO 2005 BioLight Recon (1 L)
- item code BIO2008 Disposable Glass Cuvettes (200)
- order code BIO2009 Disposable Glass Cuvettes (1000)
- order code RAW1035 10-100 µl Pipet Tips
- order code RAW1036 100-1000 µl Pipet Tips
- order code BIO2010 Extended Length Tips for Solid Phase
- order code BIO2011 Solid Phase Filter Column and Tubes (1000)
BioLight bioluminescent bacteria (reagent) is freeze-dried for extended life then stored frozen at -18°C to -20°C and is reconstituted before use. A single vial of reagent contains approximately one hundred million cells. The sample salinity is adjusted to ensure a saltwater atmosphere due to Aliivibrio fischeri being a marine bacterium. Diluent is used for dilutions as required for the test protocol.
BioLight reagents and diluents can be used with commercially available luminometer systems (Microtox®, TOXmini, LumiStox, TOXAlert, …).
Luminescent bacteria tests can be performed with field portable, bench-top and on-line analyzers.