Luminescent bacteria test_ISO 11348-3

Aliivibrio fischeri luminescent bacteria toxicity test

Biolight Aliivibrio fischeri-Vibrio fischeri Luminescent Bacteria Toxicity Test ISO11348

BIOLIGHT Aliivibrio fischeri

Acute toxicity reagents and luminometer

 

BioLight reagents are applicable for the luminescent bacteria toxicity test using the freeze dried bacteria Aliivibrio fischeri  (formerly known as Vibrio fischeri ) which has bioluminescent properties.
The inhibition of light emitted by the luminescent bacteria is the test criterion for measuring toxicity.

The BioLight Toxy all-in-one luminometer  measures the light levels of Aliivibrio fischeri to determine the toxicity of a sample.
The BioLight Acute Toxicity Reagents are standardized for use with bioassay methods described in ISO 11348-3 and ISO 21338.

Biolight Toxy Luminometer For Bioluminescent Toxicity Testing With Aliivibrio Fischeri Iso11348
Aliivibrio fischeri-Vibrio fischeri-freeze dried bacteria-ISO11348

Biolight toxy

biolight Reagents and solutions

Luminescent bacteria test
with aliivibrio fischeri

Aliivibrio fischeri  (formerly Vibrio fischeri ) is a marine, Gram negative and non-pathogen bacterium that luminesces as a natural part of its metabolism under optimal environmental conditions.

When exposed to a toxic substance, the metabolic respiratory process of this bioluminescent bacterium is disrupted, reducing light output.  This inhibition of luminescence by Aliivibrio fischeri can be measured after 5-30 minutes with a photometer (luminometer).
The reduction of light intensity measured, directly correlates with the degree of toxicity of the sample relative to the control sample.

The application of freeze-dried luminescent bacteria proves to be a useful tool for the assessment and monitoring of toxicity.
The testing combination of the BioLight Reagents and consumables with the BioLight Toxy allows for screening of 3600 chemical compounds simultaneously.

Bioassays using microbial biosensors are rapid, sensitive, reproducible and user-friendly..

Biolight Toxy benchtop luminometer for bioluminescent toxicity testing with Aliivibrio fischeri

Luminescent bacteria test method is applicable for the screening of:

chemicals

industrial effluent and process water

aqueous extracts and leachates from hazardous waste

eluates of sediment and contaminated soil

monitoring of fresh, marine and brackish water

drinking water monitoring

drilling mud or fluids

The bioluminescence inhibition bioassay with Aliivibrio fischeri  ( syn. Vibrio fischeri ) has been standardized (luminescence bacteria test ISO 11348-3 and ISO 21338) and is widely used for a variety of ecotoxicological applications and research for more than 30 years.

BioLight bioluminescent bacteria and reagents are standardized for use with:

ISO 11348-3 :  Water quality – Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test) – Part 3: Method using freeze-dried bacteria

ISO 21338 :  Water quality – Kinetic determination of the inhibitory effect of sediment, other solids and coloured samples on the light emission of Vibrio fischeri (kinetic luminescent bacteria test)

ORDER INFORMATION

  • order code BIO2019L     BioLight Toxy – Bench Top
    Includes : BioLight Instrument with on board PLC and software for both field and lab protocols.
    Cooling block to hold 12 mm cuvettes with USB connection to the unit for power
    Battery for up to 8 hours use in the field.  Wall plug for inside usage.  Rugged carrying case.
  • order code BIO2018P     BioLight Toxy – Portable
    Includes : BioLight Instrument with on board PLC and software for field protocols.
    Battery for up to 8 hours use in the field.  Wall plug for inside usage.  Rugged carrying case.
  • order code BIO2018A     Upgrade from BioLight Portable to Bench Top
    Includes :  Cooling block to hold 12 mm cuvettes with USB connection to the unit for power.
    USB for adding lab protocols to the software
  • order code BIO2020     Cooling Block for 12 mm Tubes
    Includes : Cooling block to hold 12 mm cuvettes with USB connection to the unit for power.
    This item can only be acquired when the user has purhased BIO2019L or BIO2018A.
  • order code BIO2021     Cooling Block for 17 mm Tubes (Solids Testing)
    Includes : Cooling block to hold 17 mm cuvettes with USB connection to the unit for power.
    This item can only be acquired when the user has purhased BIO2019L or BIO2018A.
  • order code BIO2006        BioLight Multi Test, Freeze Dried Bacteria (10 vials/box)
  • order code BIO2007        BioLight Single Test, Freeze Dried Bacteria (50 vials/box)
  • order code BIO2001       BioLight Recon (50 mL)
  • order code BIO2002     BioLight Diluent (1 L)
  • order code BIO2003     BioLight Solid Phase Diluent (1 L)
  • order code BIO2004     BioLight Salinity Adjusting Solution (50 mL)
  • order code BIO2005     BioLight Recon (1 L)
  • order code BIO2017      BioLight Tri-Salt Diluent (1 L)
  • item code BIO2015A        Disposable Glass Cuvettes (200)
  • order code BIO2016A        Disposable Glass Cuvettes (1000)
  • order code RAW1035      10-100 µl Pipet Tips
  • order code RAW1036      100-1000 µl Pipet Tips
  • order code BIO2010        Extended Length Tips for Solid Phase
  • order code BIO2011        Solid Phase Filter Column and Tubes (1000)

BioLight bioluminescent bacteria (reagent) is freeze-dried for extended life then stored frozen at -18°C  to -20°C and is reconstituted before use.  A single vial of reagent contains approximately one hundred million cells.  The sample salinity is adjusted to ensure a saltwater atmosphere due to Aliivibrio fischeri being a marine bacterium.  Diluent is used for dilutions as required for the test protocol.

BioLight reagents and diluents can be used with commercially available luminometer systems (Microtox®, TOXmini, LumiStox, TOXAlert, …).
Luminescent bacteria tests can be performed with field portable, bench-top and on-line analyzers.

Biolight Aliivibrio fischeri-Vibrio fischeri Luminescent Bacteria Toxicity Test ISO11348

BioLight Acute Toxicity Reagents and Biolight Toxy

Microbiotests distributes the BioLight Toxy and BioLight Reagents from the company Aqua Science , USA.

The Aqua Science team has 40+ years combined experience in the manufacturing of acute toxicity and other custom reagents used for water quality testing.  Their highly trained employees include preeminent experts in Aliivibrio fischeri production, so they can offer the highest quality option for toxicity reagent and consumables to this specific market.
The BioLight Toxy luminometer along with the BioLight Reagents and Consumables brings rapid toxicity testing to 21st century.

Their experience includes overseeing the transfer of production from the original method developer (Azur Environmental / Microbics) in 2000, production and product improvement for 11 years at Strategic Diagnostics (SDIX) and at Modern Water for 8 years.
In 2020, this entire team brought their specialized skills and comprehensive knowledge of Aliivibrio fischeri to Aqua Science where they developed BioLight, an improved formula that performs better than any competition in the market.